NavneetK Tyagi

United States
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As an award-winning research scientist, Dr. Navneet K Tyagi dedicates his life to delivering invaluable findings for the prevention and treatment of neurodegenerative diseases.
As a neuroscience researcher, Dr. Navneet k Tyagi works within a field of medicine that is always evolving. This challenge has become a way of life for Dr. Tyagi, who has undergone years of education and training. He has devoted years of his life to unfolding discoveries in protein folding and neurodegenerative diseases.

He is originally from India, but now resides in the United States, where he offers invaluable findings for treating many neurodegenerative diseases like Parkinson’s, Alzheimer’s and Lou Gehrig’s. He has researched rare cases, like the treatment for an early child onset of diabetes found in Turkish pedigree. Between contributing his knowledge to the academic world and advancing the medicinal field, Dr. Navneet Tyagi aims to excel in every aspect of his career. He will continue to delve into his field and make as many contributions as possible.

With years of experience, Dr. Navneet K Tyagi possesses a variety of valuable specialties and techniques. Below are some of his most noted ones.
Biophysical Techniques:
● Steady-state fluorescence
● Stop-flow fluorescence techniques
● Flow cytometry
● Confocal spectrophotometry
● Octet
● Mass spectrometry
● Analytical ultracentrifugation
● Circular dichroism
Protein Biochemistry:
● Polyclonal and monoclonal antibodies generation and purification
● Protein expression, purification (HPLC, UPLC, CF/DF, Unicorn 6, Soft Max Pro, AKTA) and characterization methods
● Immunogenicity assays
● Virus and virus-like particle purification
● Neutralization and attachment assay
● Immunochemical techniques
● Electrophoretic techniques
● Kinetic assays (radioactive and non-radiotactive)
● Chromatographic techniques
Molecular Biology:
● Mutagenesis
● Cloning in E. coli, yeast, insect and mammalian vectors
● Sequencing
● Cell Biology
● Protein expression and purification
● Cell culture
● Transfection
● Microinjection
● Cells
Even in his younger years, Dr. Navneet K Tyagi always knew his passion lay in genetic and biological research. In order to prepare for a career in this challenging field, he attended the CCS University in Meerut, India, where he earned his B.S. degree in Biology. He then attended IIT Roorkee University, where he received his Master’s degree in Chemistry in 1999. After this accomplishment, Dr. Tyagi realized that in order to continue his studies and knowledge in the field, he needed to leave India.

Dr. Navneet K Tyagi arrived in Dortmund Germany, where he obtained his PhD from Max Planck Institute. Following his graduation, he traveled to the United States, where he currently resides and works as a commended research associate at Yale School of Medicine. From 2006 to 2011, he studied at the university’s Howard Hughes Medical Institute. Throughout his career his techniques have led to the advancement of treatments for neurodegenerative disease, done through the manipulation of enzymes, proteins and antibodies.

Honors and Research Grants

While Dr. Navneet K Tyagi has received numerous honors, which are both commendable and impressive, the true value these honors hold is in the funding they provide to his research. The more funding he receives, the more research he can complete, which enables him to further his knowledge of protein folding and degenerative diseases. The more research he is able to complete, the better the chance of making advancements towards a cure.

Here are some of the awards and fellowships Dr. Navneet K Tyagi has received over the years:

● Research Associate Fellowship from Howard Hughes Medical Institute, Chevy Chase, Maryland, 2006-2011.
● Martin-Schmeisser Fellowship from the University of Dortmund, Germany, 2003.
● International Max Planck research in chemical biology fellowship from Max Planck Institute for Molecular Physiology in Dortmund, Germany, 2002-2005.
● Junior Research Fellowship from the Department of Science in New Delhi, India, 1999-2001.
● Passed the Graduate Aptitude Test in Engineering (GATE) in India with the rank of 100 out of 200,000 students.
In addition to his many honors, Dr. Tyagi has also taken advantage of a number of societies and foundations dedicated to raising disease awareness and treatments. These groups have enabled him to network with equally intelligent researchers in his specific field. Together, these researchers can exchange their latest findings and news, helping make advancements towards treatments and cures. Below are some of the instrumental societies Dr. Tyagi holds membership with.
● Society for Neuroscience
● Protein Society
● Yale Consulting Group
● ALS/MND Support Group Forums
● Parkinson Disease Online Research (Michael J. Fox Foundation)
● Yale Biotechnology and Pharmaceutical Society

Dr. Tyagi’s Vital Discoveries

Dr. Navneet Tyagi has used his top-tier educational background and research opportunities to bring forth brilliant discoveries in his field of work. He received his education from some of the worlds’ greatest universities in India and Europe. He then found himself learning and working among the best and most intelligent minds in the United States. Together, he and his brilliant colleagues have been able to collaborate on work and co-write invaluable journals referenced by both doctors and scholars.

Max Planck Research School

While earning his PhD in Germany at the Max Planck Research School, Dr. Tyagi was able to prove why he was chosen from such a large selection of international applicants. As an equivalent to the top ten universities in the United States, the school attracts the likes of some of the brightest students across the globe. Gaining acceptance is an impressive accomplishment in itself.

Dr. Tyagi quickly began leaving his footprint, assisting Dr. Rolf K.H. Kinne in his world-renowned research in physiology and biochemistry. Both of these were used to treat and prevent diabetes. Dr. Tyagi achieved his own personal milestone after creating a Pichia pastoris-based functional expression system for the human sodium/D-glucose cotransporter 1 protein expression and purification. In addition, he identified the sugar/inhibitor binding sites and the different conformational states of the transporter for the first time. Medical students and scholars alike regularly make use his contributions, which are also referenced in peer-reviewed journals.

Yale Medical School

As Dr. Navneet Tyagi continued to delve further into his research, he eventually began studying at Yale Medical School. There, he became research partners with Dr. Arthur Horwich, an internationally recognized scientist known for his work with molecular chaperones, protein folding and their roles in preventing and treating neurodegenerative diseases. What causes these diseases is the mutation and misfolding of the proteins made by DNA. The work of both scientists has helped develop treatments and great advancements for Alzheimer’s, ALS, type 2 diabetes, Parkinson’s, emphysema and cystic fibrosis.

Advocate for ALS

Dr. Navneet Tyagi urges people to become advocates for ALS. ALS stands for amyotrophic lateral sclerosis, the technical name for Lou Gehrig’s disease. It’s a chronic degenerative illness that starts in the brain and works its way down the spine. As it progresses, the symptoms worsen until the patient loses all control of their muscle functions. The loss of muscle control occurs because nerve cells are mutating and dying. Over time, as these vital cells continue to fail, the nerve connections that carry signals between the brain and body are destroyed.

An ALS advocate is an individual who wants to do everything and anything in his or her power to fight against the diminishing effects of Lou Gehrig’s disease. Many of these people know someone who is currently suffering of someone who has suffered from the disease. Yet, others are simply inspired to help in the fight. There are many ways advocates can contribute to the prevention and treatment of Lou Gehrig’s.
First, the advocate can become a spokesperson and deliver heartfelt speeches at events in the community.

Advocates can also organize local events like races, parties and luncheons to raise awareness and funds for ALS research. Others get involved in the political aspect of the disease, involving themselves in government functions and legislation ensuring the government is paying attention to the health care laws and insurance policies surrounding ALS. Researchers like Dr. Navneet Tyagi do their part by contributing their knowledge and discoveries.
Dr. Navneet Tyagi continues his research with Dr. Horwich, as they uncover the connection between neurodegenerative disease and protein folding. Their latest research findings were presented at the 2010 meeting on Molecular Chaperones and Stress Responses on Cold Spring Harbor, New York. They continue to make valuable findings, which will further deepen their understanding of how proteins fold and eventually get closer to finding a cure for degenerative diseases.

A major part of Dr. Navneet Tyagi’s research requires the separation of the components of various mixtures. As theses techniques are always advancing, he will continue to perfect his methods.
These methods include downstream processing, which is the recovery and purification of biosynthetic products from the natural sources like animal and plant tissue. Continuous configuration is another technique that utilizes centrifugal forces for the sedimentation of mixtures with a centrifugal device usually found in laboratories. Tangential flow filtration is also known as cross-flow filtration, with the mixture getting filtered as it travels across the surface of the filter rather than through it. Sterile filtration involves the sterilization of fluids by microfiltration using membrane filters. Additionally, Dr. Tyagi uses chromatography, a separation method where the mixture is dissolved into a fluid called the mobile stage. The mixture is then carried through a structure holding another material known as the stationary phase.

Dr. Navneet Tyagi will continue to dedicate his life to understanding neurodegenerative diseases. His research on the sodium/D-glucose cotransporter protein 1 will further help in the understanding of Type 2 diabetes and obesity.
Dr. Navneet K Tyagi is interested in developing treatment for Neurodegenerative diseases. Navneet K Tyagi is also passionate about finding treatments for rare and ultra-rare diseases.

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  • MPI Dortmund/Harvard Medicl School-Harvard University , United States

    PhD Biochemistry
    11/2001 - 09/2005
  • Indian Institute of Technology Roorkee , United States

    MS Chemistry
    07/1997 - 06/1999
  • CCS University , United States , Meerut

    BS Biology
    07/1994 - 06/1997
Career History
  • Director , United States , In my role as Director of Monoclonal Antibody Production and Process Development, I lead the cell culture group, antigen and antibody discovery, production, purification and characterization teams/projects. Technical duties include: • I am involved in high yield, scalable and cost efficient downstream process development for recombinant proteins and antibodies by using tangential flow filtration, depth & sterile filtration, and numerous modes of chromatography. • Recombinant protein and monoclonal antibody purification through AKTA systems (AKTAexpress Mab, purifier, explorer and pilot). • Protein and antibody characterization by a wide array of analytical tools. • Authoring scientific reports, providing technical support and training to different groups. • Accountable for ensuring successful contract work execution, on-time delivery, on-budget work stages and customer satisfaction.

    not defined
    05/2013 to 12/2099
  • Scientific Consulting , United States , Technology development and consulting on issues related to downstream process development for biologics, neurodegenerative diseases and protein science.

    not defined
    12/2011 to 04/2013
  • Scientist , United States , Reaction mechanism of GroEL/GroES. Extensive experience in method development for protein folding and misfolding with particular emphasis on the roles of the molecular chaperones Hsc70, Hsp110, and Hsp40 in SOD1-linked ALS disease. Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) association with an early-onset progressive multi-system neurodegenerative syndrome. • In this position I developed purification and characterization platforms for molecular chaperones, polyclonal and monoclonal antibodies, UCHL1, SOD1, MDH, MBP, Rubisco, virus and virus like particle, and radio labeled proteins. • I was involved in Downstream processing; continuous centrifugation, tangential flow filtration, depth & sterile filtration, and numerous modes of chromatography. • Authored scientific reports for publications and provided technical support to the junior members of the group. Technical duties include: • Analytics (HPLC, SDS-PAGE, IEF, CE, western blot, ELISA). • Protein purification through AKTA explorer and AKTA Pilot. • Used resin chromatography for resin screening. • Development of kinetic assays and bioconjugation protocols for enzymes.

    not defined
    02/2006 to 11/2011
  • Research Associate , Structure- Function studies of human sodium/D-glucose cotransporter 1 (hSGLT1) protein. • Developed expression and purification system for human sodium/ D-glucose cotransporter1 (hSGLT1) protein in Pichia pastoris. • Developed enzymatic assays for hSGLT1. • Determined the D-glucose and inhibitor binding sites through hSGLT1 protein by using different molecular biology and biophysical techniques, such as tryptophan scanning, photoaffinity labeling and mass spectrometric techniques (MALDI-TOF and LC-MS/ MS). • Training new team members in day-to-day operational activities.

    not defined
    11/2001 to 09/2005
Family & Friends

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Core Competencies
  • Monoclonal Antibody Engineering
  • Antibody & protein characterization
  • Downstream process development
  • Neurodegenerative disease
  • Protein Science
  • Drug Discovery
  • Cell Biology
  • Protein purification
  • Ultra-rare diseases
  • Scientific consulting
Greatest Attributes

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Worst Failures in Business

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